Publications
Book Edition:
"The Pollen Tube - A Cellular and Molecular Perspective" (Springer-Verlag, ISBN-10 3-540-31121-1)
Peer-reviewed publications:
64. Vaz Dias, F., Serrazina, S., Vitorino, M., Marchese D., Heilmann, I., Godinho, M., Rodrigues, M., Malhó R. (2019) A role for Diacylglycerol Kinase 4 in signaling crosstalk during Arabidopsis pollen tube growth. New Phytologist. doi.org/10.1111/nph.15674.
63. Malhó R. (2018) Expand, colonize, consolidate! Nature Plants. 4: 861-862. https://doi.org/10.1038/s41477-018-0297-4.
62. Gonçalo Laureano; Joana Figueiredo; Ana R Cavaco; Bernardo Duarte; Isabel Caçador; Rui Malhó; Marta Sousa Silva; Ana R Matos; Andreia Figueiredo (2018) The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola. Scientific reports. 8: 14538. DOI:10.1038/s41598-018-32559-z
61. Joana Dias, Sandra Aguiar, Diane Pereira, Ana André, Lurdes Gano, João D. G. Correia, Belmira Carrapiço, Barbara Rutgen, Rui Malhó, Conceição Peleteiro, João Goncalves, Cecília Rodrigues, Solange Gil, Luís Tavares, Frederico Aires-da-Silva (2018) The histone deacetylase inhibitor panobinostat is a a promising therapeutic agent for treatment of canine diffuse large B-cell lymphoma. Oncotarget. 9: 28586-28598
60. Sebastiana M., Silva A.B., Matos A.R., Alcântara A., Silvestre S., Malhó R. (2018) Ectomycorrhizal inoculation with Pisolithus tinctorius reduces stress induced by drought in cork oak. Mycorrhiza 28: 247-258. doi.org/10.1007/s00572-018-0823-2
59. Figueiredo, J., Costa, G., Maia, M.R.G, Paulo, O. Malhó, R., Sousa, M., Figueiredo, A. (2016) Revisiting Vitis vinifera subtilase gene family: a possible role in grapevine resistance against Plasmopara viticola. Frontiers in Plant Science 25: 1783. doi:10.3389/fpls.2016.01783
58. L. Saavedra, R. Catarino, T. Heinz, I. Heilmann, M. Bezanilla, R. Malhó (2015) Phosphatase and tensin homolog (PTEN) is a growth repressor of both rhizoid and gametophore development in the moss Physcomitrella patens. Plant Physiology 13: 1197- doi.org/10.1104/pp.15.01197
57. Malhó, R., Serrazina, S., Saavedra, L. Vaz Dias, F., Rehman R., (2015) Ion and lipid signalling in apical growth – a network regulating morphogenesis and responses to extracellular cues. Frontiers in Plant Science 6: 816. doi: 10.3389/fpls.2015.00816.
56. Serrazina, S., Vaz Dias, F., Malhó R. (2014) Characterization of FAB1 phosphatidylinositol kinases in Arabidopsis pollen tube growth and fertilization. New Phytologist. DOI: 10.1111/nph.12836
55. Tunc-Ozdemir*, M., Rato*, C., Brown, E., Rogers, S., Mooneyham, A., Frietsch, S., Myers, C.T., Poulsen, L.R., Malhó, R., Harper, J.F. (2013) Cyclic nucleotide gated channels 7 and 8 are essential for male reproductive fertility. (*) contribuição identica na autoria. PLoS One. 8(2):e55277. doi: 10.1371/journal.pone.0055277.
54. Silva, S., Alves, A.C.S., Patel, D., Malhó, R., Soutar, A.K., Bourbon, M. (2012) In vitro functional characterization of missense mutations in the LDLR gene. Atherosclerosis. 225: 128-134. DOI: 10.1016/j.atherosclerosis.2012.08.017
53. Aires da Silva F., Li, M., Rato, S., Maia S., Malhó R., Warren, K., Harrich, D., Craigie R., Barbas III C., Goncalves J. (2012) Recombinant rabbit single-chain antibodies simultaneously bind to the catalytic and C-terminal domains of HIV-1 integrase protein and strongly inhibit HIV-1 infection. Biotechnology and Applied Biochemistry 59: 353-366. DOI: 10.1002/bab.1034
52. Saavedra L., Mikami K., Malhó R., Sommarin M. (2012) PIP kinases and their role in plant tip growing cells. Plant Signalling & Behavior 7(10): 1302-1305. DOI: 10.4161/psb.21547
51. Rehman R., Ângelo Silva P., Malhó R. (2011) Localization of Arabidopsis SYP125 syntaxin in the plasma membrane sub-apical and distal zones of growing pollen tubes. Plant Sig. & Behav 6(5): 665-670.
50. Santos R., Miguel S., Malhó R., Maycock C., Fevereiro P., Oliva A. (2010) The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture. Journal of Nanobiotechnology 8:24
49. Ângelo Silva P., Rehman R., Rato C., Di Sansebastiano G-P., Malhó R. (2010) Asymmetric localization of Arabidopsis syntaxins at the pollen tube apical and sub-apical zones is involved in tip growth. BMC Plant Biol. 10: 179
48. Pedroso N., Matias A.C., Cyrne L., Antunes F., Borges C., Malhó R., Almeida R.F.M, Herrero E., Marinho H.S. (2009) Modulation of plasma membrane lipid profile and microdomains by H2O2 in Saccharomyces cerevisiae. Free Radical Biol & Medicine. 46: 289-298
47. Sousa E., Kost B., Malhó R. (2008) Arabidopsis Phosphatidylinositol-4-Monophosphate 5-Kinase 4 regulates pollen tube growth and polarity by modulating membrane recycling. Plant Cell 20: 3050-3064
46. Fortes A.M., Santos F., Figueiredo A., Choi Y., Silva M.S., Sousa L., Pessoa F., Acioli B., Sebastiana M., Palme K., Malhó R., Verpoorte R., Pais M.S. (2008) Transcriptional and metabolic profiling during organogenic nodule formation in hop. BMC Genomics 9: 445
45. Banha J., Marques L., Oliveira R., Paixão E., Martins M.F., Malhó R., Penque D., Costa L. (2007) Ceruloplasmin expression by human peripheral blood lymphocytes: a new link between immunity and iron metabolism. Free Radical Biol & Medicine. 44: 483-492
44. Garcês H.M.P., Champagne C.E.M., Townsley B.T., Park S., Malhó R., Pedroso M.C., Harada J.J., Sinha N.R. (2007) Evolution of asexual reproduction in leaves of the genus Kalanchoë. Proc. Natl. Acad. Sci. USA 104: 15578-15583
43. Marques L., Banha J., Penque D., Barreto M., Malhó R., Costa L. (2007) Ceruloplasmin and ferroportin are expressed at the surface of human peripheral blood lymphocytes. Amer. J. Hematology 82: 549-549.
42. Rodrigues* C., Deeks* M.J., Dimmock S., Ketelaar T., Maciver, S.K., Malhó R., Hussey P.J. (2007) Arabidopsis CAP1, a key regulator of actin organisation and development. (*) these authors contributed equally. J. Cell Sci. 120: 2609-2618.
41. Malhó R., Kaloriti D., Sousa E. (2006) Calcium and rhythms in plant cells. Biological Rhythms Research – Plant Chronobiology. Eds. Waterhouse J., Wagner E., Rietveld, W. 37: 297-314.
40. Castanho Coelho P., Malhó, R. (2006) Correlative analysis of apical secretion and [Ca2+]C in pollen tube growth and reorientation. Plant Signaling & Behavior. 1: 152-157
39. Fortes, A.M., Miersch, O., Lange, P.R., Malhó,R., Testillano, P.S., Risueño,M.C., Wasternack, C., Pais, M.S. (2005) Expression of Allene Oxide Cyclase and Accumulation of Jasmonates During Organogenic Nodule Formation from Hop (Humulus lupulus var. Nugget) Internodes. Plant and Cell Physiology 46: 1713-1723.
38. Zheng H., Camacho L., Batoko H., Wee E., Legen J., Leaver C., Malhó, R., Hussey P., Moore I. (2005) A RabE GTPase mutant acts downstream of the RabD subclass in biosynthetic membrane traffic to the plasma membrane. Plant Cell. 17: 2020-2036
37. Malhó R., Liu Q., Monteiro D., Rato C., Camacho L., Dinis A. (2006) Signalling pathways in pollen germination and tube growth. Protoplasma. 228: 21-30
36. Monteiro D., Castanho Coelho P., Rodrigues C, Camacho L., Quader H., Malhó, R. (2005) Modulation of endocytosis in pollen tube growth by phosphoinositides and phospholipids. Protoplasma. 226: 31-38.
35. Monteiro D., Liu Q., Lisboa S., Scherer G.E.F., Quader H., Malhó R. (2005) Phosphoinositides and phosphatidic acid regulate pollen tube growth and reorientation through modulation of [Ca2+]c, and membrane secretion. J. Exptl. Bot. 56: 1665-1674.
34. Deeks M.J., Kaloriti D., Davies B., Malhó R., Hussey P.J. (2004) Arabidopsis NAP1 Is Essential for Arp2/3-Dependent Trichome Morphogenesis. Curr. Biol. 14: 1410-1414.
33. Rato C., Monteiro D., Hepler P.K., Malhó R. (2004) Calmodulin activity and cAMP signalling modulate growth and apical secretion in pollen tubes. The Plant Journal. 38: 887-897.
32. Carvalho-Oliveira I., Efthymiadou A., Malhó R., Tzetis M., Kanavakis E., Amaral M.D., Penque M.D. (2003) CFTR Localization in Native Airway Cells and Cell Lines Expressing Wild-type or F508del-CFTR by a Panel of Different Antibodies. J. Histochem. Cytochem. 52: 193-204.
31. D’Angeli S., Malhó R., Altamura M.M. (2003) Low temperature sensing by olive tree: calcium signaling and cold acclimation. Plant Sci. 165: 1303-1313.
30. Camacho L., Malhó R. (2003) Endo-Exocytosis in the pollen tube apex is differentially regulated by [Ca2+]c and GTPases. J. Exptl. Bot. 54: 83-92.
29. Gonçalves J., Silva F., Freitas-Vieira A., Santa-Marta M., Malhó R., Yang, X., Gabuzda D., Barbas III C. (2002) Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. J. Biol. Chem. 277: 32036-32045.
28. Caperta A., Neves N., Morais-Cecílio L., Malhó R., Viegas W. (2002) Genome restructuring in rye affects the expression, organization and disposition of homologous rDNA loci. J. Cell Sci. 115: 2839-2846.
27. Trewavas A.J., Rodrigues C., Rato C., Malhó R. (2002) Cyclic nucleotides: the current dilemma! Curr. Op. Plant Biol. 5: 425-429.
26. Moutinho A., Hussey P., Trewavas A.J., Malhó R. (2001) The role of cAMP and adenylate cyclase in pollen tube growth and reorientation. Proc. Natl. Acad. Sci. USA. 98: 10481-10486.
25. Moutinho A., Camacho L., Pais M.S., Trewavas A.J., Malhó R. (2001) Antisense suppression of protein function in living pollen tubes. Sex. Plant Reprod. 14: 101-104.
24. de Ruitjer N., Malhó R. (2000) Plant and fungal tip growth: where do we go from here ? Trends in Plant Science. 5: 453-454.
23. Penque D., Mendes F., Beck S., Farinha C., Pacheco P., Nogueira P., Lavinha J., Malhó R., Amaral M.D. (2000) Cystic Fibrosis F508del-patients have apically localized CFTR in a reduced number of airway cells. Laboratory Investigation. 80: 857-868.
22. Xavier, J.B., Malhó, R., Reis, A.M., Almeida, J.S. (2000) Description of Biofilm Formation by Determination of Developmental Axis. Water Science and Technology. 41: 121-127.
21. Camacho L., Parton R.M., Trewavas A.J., Malhó R. (2000) [Ca2+]c distribution and oscillations in pollen tubes with confocal microscopy. A comparison of different dyes and loading methods. Protoplasma 212: 162-173.
20. Malhó R. Camacho L., Moutinho, A. (2000) Signaling pathways in pollen tube growth and reorientation. Annals of Botany. 85 (suppl A): 59-68.
19. Malhó R. (1999) Coding information in plant cells: the multiple roles of Ca2+ as a second messenger. Plant Biol. 1: 487-494.
18. Trewavas A.J., Malhó R. (1998) Ca2+ Signalling in plant cells: the big network. Curr. Op. Pl. Biol. 1: 428-433.
17. Penque D., Gomes A., Mock M., Farinha C., Malhó R., Demolombe S., Escande D., Lavinha J., Amaral M.D. (1998) Cystic Fibrosis: cellular characterization of A561E Portuguese mutation. Eur. J. Hum. Gen. 6(S1): P5016.
16. Moutinho A., Trewavas A.J., Malhó R. (1998) Relocation of a Ca2+-Dependent Protein Kinase activity during Pollen Tube Reorientation. Plant Cell. 10: 1499-1510.
15. Moutinho A., Love J., Trewavas A.J., R. Malhó R. (1998) Distribution of Calmodulin Protein and mRNA in Growing Pollen Tubes. Sex. Plant Reprod. 11: 131-139.
14. Malhó R., Moutinho A., Van der Luit A., Trewavas A.J. (1998) Spatial characteristics of Ca2+ signalling; the calcium wave as a basic unit in plant cell calcium signalling. Phil. Trans. R. Soc. B 353, 1463-1473.
13. Malhó R. (1998) Pollen Tube Guidance: the long and winding road. Sex. Plant Reprod. 11: 242-244.
12. Malhó R. (1998) Role of 1,4,5-Inositol Triphosphate-Induced Ca2+ Release in Pollen Tube Orientation. Sex. Plant Reprod. 11: 231-235
11. Malhó R. (1998) Expanding tip-growth theory. Trends in Plant Science. 3: 40-42.
10. Trewavas A.J., Malhó R. (1997) Signal perception and transduction. The origin of the phenotype. The Plant Cell. 9: 1181-1195.
9. Parton R., Fischer S., Malhó R., Papasouliotis O., Jelitto T., Leonard T., Read N. (1997) Pronounced gradients in cytoplasmic pH are not required for tip growth in plant and fungal cells. J. Cell Sci. 110: 1187-1198.
8. Malhó R., Trewavas A.J. (1996) Localized Apical Increases of Cytosolic Free Calcium Control Pollen Tube Orientation. The Plant Cell 8: 1935-1949.
7. Feijó J.A., Malhó R., Obermeyer G. (1995) Ion dynamics and its possible role during in vitro pollen germination and tube growth. Protoplasma 187: 155-167.
6. Malhó R., Read N., Trewavas A.J., Pais M.S. (1995) Calcium channel activity during pollen tube growth and reorientation. The Plant Cell 7: 1173-1184.
5. Malhó R., Read N., Pais M.S., Trewavas A.J. (1994) Role of cytosolic free calcium in the reorientation of pollen tube growth. The Plant Journal 5: 331-341.
4. Read N.D., Allan W.T.G., Knight H., Knight M.R., Malhó R., Rusell A., Shacklock P., Trewavas A.J. (1992) Imaging and measurement of cytosolic free calcium in plant and fungal cells. J. Microscopy 165: 586-616.
3. Feijó J.A., Malhó R., Pais M.S. (1992) A cytochemical study on the role of ATPases during pollen germination in Agapanthus umbellatus L´Her. Sexual Plant Reproduction 5: 138-145.
2. Malhó R., Pais M.S. (1992) Kinetics and Hydrodynamics of Agapanthus umbellatus Pollen Tube. A structural and stereological Study. Sexual Plant Reproduction. 5: 163-168.
1. Malhó R., Feijó J.A., Pais M.S. (1992) Effect of electrical fields and external ionic currents on pollen tube orientation. Sexual Plant Reproduction 5: 57-63.
Book chapters:
1. Malhó R. (1998) The role of calcium and associated proteins in tip growth and orientation. In: Fertilization in higher plants. Molecular and Ctyological Aspects. Eds M. Cresti, G. Cai and A. Moscatelli. Springer-Verlag. Pp 253-270.
2. Malhó R. (2000) Coding information in plant cells using Ca2+ as a second messenger - The cell as the minimum unity for complexity. "Advanced Studies in Plant Biology", Universidade de Geneva. Eds. H. Greppin, C Penel & R. Degli Agosti. Pp 311-339.
3. Malhó R., Camacho L., Moutinho, A. (2002) Imaging ions and molecules in living plant cells. In: Molecular Plant Biology Volume two: A practical approach. Eds P. Gilmartin & C. Bowler. Oxford Univ. Press. ISBN 0-19-963818-7
4. Malhó R., Moutinho A. (2001) A cAMP pathway in pollen tube growth and reorientation. In: Cell Biology of Plant and Fungal Tip Growth. NATO ASI Series vol 328. Eds A. Geitmann, M. Cresti and I.B.Heath. Pp 95-104.
5. Malhó R., Camacho L. (2002) Ca2+ signalling systems in plants. The cell as the minimum unity for complexity. In: "ADVANCES IN PLANT PHYSIOLOGY", Volume 5. Eds. Hemantaranjan A. Institute of Agricultural Sciences, Varanasi, India. Pp 179-203.
6. Malhó R., Camacho L. (2004) Signalling the cytoskeleton in pollen tube germination and growth. In: The Plant Cytoskeleton in Cell Differentiation and Development. Eds. Hussey P.J., Ann. Plant Review Series, Blackwell Publishers, UK. Pp 240-264.
7. Malhó R., Castanho Coelho P., Pierson E., Derksen J. (2005) Endocytosis and membrane recycling in pollen tubes. In: The Plant Endocytosis. Eds. Samaj, J., Baluska, F., Menzel, D., Springer-Verlag, Germany. Pp 277-291.
Others (Proceedings):
1. Gonçalves J., Silva F., Freitas-Vieira A., Santa-Marta M., Malhó R., Yang, X., Gabuzda D., Barbas III C. (2002) Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits viral replication. XIV international aids conference: basic sciences pp 219-226
2. Costa M.J.L., Malhó R, Goncalves J., Ferreia G.N.M., Aires-Barros M.R. (2005) Development of a 293t cell line for the production of virus like particles in bioreactors Animal Cell Technology Meets Genomics. 18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT). Springer-Verlag.
3. Deeks M.J., Kaloriti D., Machesky L.M., Malhó R., Davies, B., Hussey P.J. (2005) Actin nucleators in plants. Comparative biochemistry and physiology 141(3): S320-S321
4. R. Malhó, P. Castanho Coelho, M. Ovecka, I. Lichtscheidl (2005) Structural and dynamic aspects of pollen tube guidance: can actin deliver? Comparative biochemistry and physiology 141(3): S324-S324
5. Bastos, A.E.P., Khmelinskaia, A., Scolari, S., Malhó, R., Herrmann, A., Marinho, H.S., Almeida, R.F.M. (2012) Sterol Properties Required for Microdomain Formation: From Model Systems to Living Yeast and Mammalian Cells. Biophysical Journal. 102: 298A-298A.
6. M.M. Tenreiro, R. Malhó, M. Silveira, M.L. Correia, M.A. Brito (2016) A NOVEL TOOL TO MONITOR ANGIOGENESIS IN BONE MARROW SMEARS: DETECTION AND QUANTIFICATION OF ENDOTHELIAL PROGENITOR CELLS BY MULTIPLE-LABELING IMMUNOFLUORESCENCE ANALYSIS. Haematologica 101: 782-783, Suppl 1.
7. Tenreiro MM, Malhó R, Silveira M, Correia ML, Brito MA. (2016) Assessment of endothelial progenitor cells in bone marrow smears by multiple-labelling immunofluorescence analysis: a novel tool to monitor angiogenesis. Archives of Anatomy 4: 69-70A.
8. Khmelinskaia, A., Antunes, CA., Bastos, AEP., Scolari, S., Lobo, GMDS., Marques, JMT., Malhó, R., Herrmann, A., Marinho, HS., de Almeida, RFM (2017) Liquid ordered phase formation by membrane sterols was required for eukaryotic membrane development. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS Vol 46 Suppl 1, S254-S254.
9. Vicente R, Custódio-Santos T, Molnár K, Haskó J, Malhó R, Wilhelm I, Krizbai I, Videira M, Brito MA. (2018) Breast cancer brain metastasization: comparison of hippocampal and cerebellar patterns. Morphology 53 (Suppl 3): 108A.